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Histology Services/Equipment

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The Histology Core of the Department of Anatomy and Cell Biology at the Indiana University School of Medicine provides histological services for basic science (non-clinical) research. Both mineralized (plastic embedded) and soft tissue (paraffin embedded) specimens can be prepared. Services that are available are:


Paraffin Embedding and Sectioning

  1. De-mineralization of mineralized tissue (if necessary)
  2. Processing of specimens through paraffin embedding
  3. Thin sectioning of paraffin-embedded tissue
  4. Staining of paraffin sections

 

* For routine assessment, slides are stained with hematoxylin and eosin (HE; Harris hematoxylin, regressive method. Offered specialized stains include:

Plastic (methyl methacrylate) Embedding and Sectioning

  1. Processing of specimens through plastic (methyl methacrylate; MMA) embedding
  2. Thick sectioning of cortical bone
  3. Thin sectioning of trabecular (cancellous) bone
  4. Staining of plastic sections

 

** Cover-slipped unstained sections for analysis of fluorochromes (dynamic histomorphometry) are provided at no charge. Offered specialized stains (for static histomorphometry) include:

Equipment

Shandon Pathcentre Tissue Processor (for paraffin and plastic)

DAPCO Model 5831 vacuum ovens (N=2)

Leice DM 3000 photomicroscope with LAS imaging software

 

Submissions

Materials for processing can be dropped off on the bench opposibe the lab door (MS-5045G). All submissions need to fill out a owrk order (WO) form which can be found above the bench or downloaded here:

[Formalin-fixed} tissue for either plastic or paraffin embedding should be submitted immersed in 70% ethanol (EtOH), not formalin. Specimens not submitted in histology cassettes will be charged for cassettes (see Fee Schedule), if appropriate.

N.B. Cassettes must be labeled with either pencil or solvent-resistant markers (see list below). Labeling done with sharpies or similar ink pens will come off during processing. Approved marking pens include:

-Blocks for re-cutting and slides for staining can be submitted as is.

-The designated contact person will be notified by email when the work order is complete.

-Questions concerning submissions should be submitted to Keith Condon (kcondon@iupui.edu).

Department of Anatomy & Cell Biology | IU School of Medicine | 635 Barnhill Drive, MS 5035 | Indianapolis, IN 46202 | (317) 274-7494