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The Organ Lab at IUSM studies the relationship between bone and muscle mechanics at the whole-organ level, and how tissue-level mechanisms influence whole-organ function. We utilize numerous in vivo model systems to understand how disease, exercise, and pharmaceutical intervention influence bone and muscle structure, cellular activity, and biomechanical properties. We study these properties in the context of chronic kidney disease, osteogenesis imperfecta, and normal postnatal ontogeny, using a variety of techniques: imaging (CT, DXA, X-ray), histology (static and dynamic histomorphometry), muscle electrophysiology (in vivo ankle torque, in vitro whole muscle contractility), and bone mechanical testing (bending, compression, reference point indentation). The Organ Lab is funded by the NIH, IUSM/Showalter Research Trust, and the Indiana University Collaborative Reseach Grant Fund.

Jason Organ

    Name: Jason Organ, PhD

    Role: PI

    Email: jorgan@iupui.edu

    Office: MS 5035

 

    Name: Kelly Biro, BS

    Role: Research Technician

    Email: kelbiro@iupui.edu

    Office: MS 531


    Name: Lexie Stachel, BS

    Role: Research Technician

    Email: astachel@iupui.edu

    Office: MS 531

 

    Name: Lexy Chavez

    Role: Pike High School / Project SEED Intern

 


LAB ALUMNI

Mark Humphrey, MS (Saint Louis University, 2008-2010)

Colleen Steinkoenig, MS (Saint Louis University, 2008-2010)

Naomi Schmalz, MS (Saint Louis University, 2010-2012)

Chetna Sethi, BS (Saint Louis University Forensics Internship, 2010-2012)

Jeffery Joll (IUPUI - LHSI Internship, 2013-2014)

Jeremy Mihajlovich (IUPUI - LHSI Internship, 2013-2014)

Andrew Srisuwananukorn, BS (IUSM - SRPinAM Internship, 2014)

Paige Price (IUSM - Project STEM Internship, 2014)

Wiaam Elkhatib (IUPUI - LHSI Internship, 2014-2015)

Ben Vickery (IUPUI - LHSI Internship, 2014-2015)

Joe Rupert (IUSM - IBMG Rotation Student, 2014)

Cayli Meizel-Lambert (IUSM - IBMG Rotate Student, 2015)

Adam Myers-White (SRPinAM Internship, 2015)

Andrew Guitierrez (Project STEM Internship, 2015)

Gregory Monnin (IUPUI - LHSI Internship, 2015-2016)

Lab Projects


1. Examining the effects of multidirectional mechanical loading on the mechanical properties of the musculoskeletal system.

2. Enhancing musculoskeletal mechanical properties in Osteogenesis Imperfecta (funded by Showalter Research Trust).

3. Modulating the cellular and structural mechanisms underlyiung craniofacial development in Osteogenesis Imperfecta (funded by Indiana University Collaborative Research Grant Fund).

4. Enhancing musculoskeletal mechanical properties in Chronic Kidney Disease (previously funded by National Skeletal Muscle Research Center).


Lab Techniques

 

Imaging
Assessment of cortical bone geometry and cancellous bone architecture is an essential component of our lab. We use several techniques for these assessments including digital radiography, dual energy x-ray absorptiometry, peripheral quantitative computed tomograph, micro-computed tomography.

Major equipment, shared among many labs at IUSM, include:
X-ray: Micro Photonics PiXarray 100 digital x-ray machine
DEXA: PixiMus II densitomiter (Lunar Corp)
pQCT Norland Stratec XCT Research SA+, XCT-2000, and XCT-3000 pQCT machines
MicroCT: Skyscan 1172 and 1176 high-resolution desktop micro-computed tomography machines and all associated software.


Histology
Assessment of tissue cellular dynamics and damage represent core techniques of most of our studies. We use several techniques for these assessments including:
Dynamic histomorphometry to assess mineral apposition rate, minerlaizing surface, and bone formation rate
Static histomorphometry to assess osteoblast/osteoid, osteoclast and osteocyte parameters

Major equipment in our histology laboratory, that services many labs at IUSM, includes:
Microtomes: Reichert-Jung 2050 Supercut; Jung 820 Histocut (2); Reichert-Jung Polycut microtome
Sectioning saws: Buehler Isomet Low Speed sectioning saws (2); DDK diamond wire sectioning saws (5)
Processors: Shandon automatic processor (PathCenter, Shandon); Embedding Center (Tissue-tek, Sakura); automatic stainer
Microscopes:
Nikon Optiphot research microscope for brightfield, epifluorescene
LEICA research photomicroscope with brightfield
Image Analysis Systems: Bioquant Osteo image analysis system with CCD camera attachment. 
The laboratory also has full access to a Reichert-Jung cryostat, several ultramicrotomes, and equipment for electron microscopy.

 

Mechanics
The ultimate measure of any intervention is how it affects the mechanical properties. We use several techniques for assessing the mechanical properties of bone including:
Compression testing of vertebra
Reduced platen compression (RPC) of trabecular bone
3-point bending of long bones and ribs
4-point bending of cortical beams
Compression/Shear testing of femoral neck
Reference point indentation (in vivo and ex vivo)

We also focus on muscle functional properties such as contractile force, half relaxation time, and fatigability (in vivo and ex vivo).

Major equipment in our lab, as well as that housed in the Dept of Biomedical Engineering, includes:
Low force (<1000 N) testing machine (Test Resources, 100P225)
High force (>1000 N) testing machine (MTS, Alliance RT/5 and Bionix)
Reference point indenter (BioDent H, Active Life Technologies)

Whole animal muscle tester (3-in-1 1300A, Aurora Scientific)



REFEREED PUBLICATIONS IN RESEARCH:

  1. Organ JM, Teaford MF, Larsen CS. 2005. Dietary inferences from dental occlusal microwear at Mission San Luis de Apalachee. Am J Phys Anthropol 128:801-811. PMID: 16134151. 
  2. Organ JM, Ruff CB, Teaford MF, Nisbett RA. 2006. Do mandibular cross-sectional properties and dental microwear give similar dietary signals? Am J Phys Anthropol 130:501-507. PMID: 16425187. 
  3. Organ JM, Ward CV. 2006. Contours of the hominoid lateral tibial condyle with implications for Australopithecus. J Hum Evol 51:113-127. PMID: 16563467.
  4. Organ JM, Teaford MF, Taylor AB. 2009. Functional correlates of fiber architecture of the lateral caudal musculature in prehensile and nonprehensile tails of the Platyrrhini (Primates) and Procyonidae (Carnivora). Anat Rec 292:827-841. PMID: 19402068.
  5. Sylvester AD, Organ JM. 2010. Curvature scaling in the medial tibial condyle of large bodied hominoids. Anat Rec 293:671-679. PMID: 20235323.
  6. Organ JM, DeLeon VB, Wang Q, Smith TD. 2010. From head to tail: new models and approaches in primate functional anatomy and biomechanics. Anat Rec 293:544-548. PMID: 20235310.
  7. Organ JM. 2010. Structure and function of platyrrhine caudal vertebrae. Anat Rec 293:730-745. PMID: 20235328.
  8. Organ JM, Muchlinski MN, Deane AS. 2011. Mechanoreceptivity of prehensile tail skin varies between ateline and cebine primates. Anat Rec 294:2064-2072. PMID: 22042733.
  9. Organ JM, Lemelin P. 2011. Tail architecture and function of Cebupithecia sarmientoi, a Middle Miocene platyrrhine from La Venta, Colombia. Anat Rec 294:2013-2023. PMID: 22042718.
  10. Gallant MA, Brown DB, Organ JM, Allen MR, Burr DB. 2013. Reference-point indentation correlates with bone toughness assessed using whole-bone traditional mechanical testing. Bone 53:301-305. PMCID: PMC3563255. http://hdl.handle.net/1805/4624.
  11. Patel BA, Ruff CB, Simons ELR, Organ JM. 2013. Humeral cross-sectional shape in suspensory primates and sloths. Anat Rec 296:545-556. PMID: 23408647.
  12. Aref M, Gallant MA, Organ JM, Wallace JM, Newman CL, Burr DB, Brown DM, Allen MR. 2013. In vivo reference point indentation reveals positive effects of raloxifene on mechanical properties following 6 months of treatment in skeletally mature beagle dogs. Bone 56:449-453. PMCID: PMC3873633. http://hdl.handle.net/1805/3375.
  13. Moe SM, Chen NX, Newman CL, Gattone II VH, Organ JM, Chen X, Allen MR. 2014. A comparison of calcium to zoledronic acid for improvement of cortical bone in an animal model of CKD. J Bone Miner Res 29:902-910. PMCID: PMC3940692. http://hdl.handle.net/1805/3644.
  14. Allen MR, Newman CL, Smith E, Brown DM, Organ JM. 2014. Variability of in vivo reference point indentation in skeletally mature inbred rats. J Biomech 47:2504-2507. PMCID: PMC4096655. http://hdl.handle.net/1805/4299.
  15. Deane AS, Russo GA, Muchlinski MN, Organ JM. 2014. Caudal vertebral body articular surface morphology correlates with functional tail use in anthropoid primates. J Morphol 275:1300-1311. PMID: 24916635. http://hdl.handle.net/1805/4533.
  16. Moe SM, Chen NX, Newman CL, Organ JM, Kneissel M, Kramer I, Gattone II VH, Allen MR. 2015. Anti-sclerostin antibody treatment in a rat model of progressive renal osteodystrophy. J Bone Miner Res 30:499-509. PMCID: PMC4333005. http://hdl.handle.net/1805/5544.
  17. Lee DY, Wetzsteon RJ, Zemel BS, Shults J, Organ JM, Foster BJ, Herskovitz RM, Foerster DL, Leonard MB. 2015. Muscle torque relative to cross-sectional area and the functional muscle-bone unit in children and adolescents with chronic disease. J Bone Miner Res 30:575-583. PMCID: PMC4532328.
  18. Rupert JE, Rose JA, Organ JM, Butcher MT. 2015. Forelimb muscle architecture and myosin isoform composition in the groundhog (Marmota monax). J Exp Bio 218:194-205. PMID: 25452499.
  19. Srisuwananukorn A, Allen MR, Brown DM, Wallace JM, Organ JM. 2015. In vivo reference point indentation measurement variability in skeletally mature inbred mice. BoneKEy Reports 4(712):1-5. PMCID: PMC4478874
  20. Organ JM, Srisuwananukorn A, Price P, Joll JE, Biro KC, Rupert JE, Chen NX, Avin KG, Moe SM, Allen MR. 2016. Reduced skeletal muscle function is associated with decreased fiber cross-sectional area in the Cy/+ rat model of progressive kidney disease. Nephrol Dial Transplant 31:223-230. PMID: 26442903.
  21. Allen MR, McNerny EMB, Organ JM, Wallace JM. 2015. True gold or pyrite: a review of reference point indentation for assessing bone mechanical properties in vivo. J Bone Miner Res 30:1539-1550.

 

REFEREED PUBLICATIONS IN TEACHING:

  1. Organ JM. 2012. Anterior compartment of leg. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT. (anatomyone.com)
  2. Organ JM. 2012. Anterior compartment of thigh and femoral triangle. In: Morton D, editor. AnatomyOne, Amirsys: Salt Lake City, UT.
  3. Organ JM. 2012. Arteries: lower limb. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  4. Organ JM. 2012. Deep back muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  5. Organ JM. 2012. Foot. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  6. Organ JM. 2012. Foot muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  7. Organ JM. 2012. Gait. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  8. Organ JM. 2012. Hip joint. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  9. Organ JM. 2012. Hip muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  10. Organ JM. 2012. Knee joint and popliteal fossa. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  11. Organ JM. 2012. Lateral compartment of leg. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  12. Organ JM. 2012. Leg muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  13. Organ JM. 2012. Lower limb. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  14. Organ JM. 2012. Lower limb muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  15. Organ JM. 2012. Medial compartment of thigh. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  16. Organ JM. 2012. Posterior compartment of leg and tarsal tunnel. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  17. Organ JM. 2012. Posterior compartment of thigh. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  18. Organ JM. 2012. Spinal nerve anatomy. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT. 
  19. Organ JM. 2012. Superficial back muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  20. Organ JM. 2012. Superficial structures of lower limb. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  21. Organ JM. 2012. Thigh muscles. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  22. Organ JM. 2012. Veins: lower limb. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.
  23. Organ JM. 2012. Vertebral column. In: Morton D, editor. AnatomyOne, Amirsys/Elsevier: Salt Lake City, UT.

Kelly Biro

2016 - Woodrow Wilson Teaching Fellowship

 

Wiaam Elkhatib

2016 - IUPUI Top 10 student award

 

Jeffery Joll

2015 - American Association of Anatomists Undergraduate Poster Finalist

2016 - IUPUI Top 10 student award

 

Jason Organ

2006 - American Association of Physical Anthropologists Mildred Trotter Prize for Mineralized Tissue Research

2011 - American Association of Anatomists Keith & Marian Moore Young Anatomists Publication Award

2011 - American Association of Anatomists Young Faculty Participation Award

2013 - American Association of Anatomists Short-Term Visiting Scholarship


Andrew Srisuwananukorn

2014 - 3rd Place Presentation - IUSM Student Research Program in Academic Medicine

 

Ben Vickery

2016 - IUPUI Top 10 student award


2016

- Jason Organ was elected to serve a 3-year term on the Board of Directors of the American Association of Anatomists.

- Jason Organ has partnered with Drs. Krista Hoffmann-Longtin and Jonathan Rossing (Communication Studies), and Mel Wininger (English) to develop a graduate minor in Science Communication at IUPUI and IUSM. This project is funded by the IUPUI Center for Teaching and Learning Curriculum Enhancement Grant program.

- The Organ Lab won an Indiana University Collaborative Research Grant with Dr. Rachel Menegaz in the IU School of Dentistry to study the craniofacial dysmorphology in a mouse model of Osteogenesis Imperfecta!


MENTORING

The Organ Lab participates in a number of mentoring programs at IUSM and IUPUI. If you are interested in learning about the various graduate study and internship programs that place students in our lab, you can find information here:

Graduate Studies Programs

Internship Programs

 

SCIENCE OUTREACH

The Organ Lab recognizes the importance of public science education and making our science accessible to the public. To this end, we are actively engaged in public outreach of science through a number of avenues.

Science Blogging

  • Eatlemania - Internet home of the dermestid (flesh-eating) beetle colony in the Organ Lab @ IUSM
  • THE 'SCOPE - Highlighting the science behind popular culture

Partnerships with Science Outreach Initiatives

Coming soon...

Department of Anatomy & Cell Biology | IU School of Medicine | 635 Barnhill Drive, MS 5035 | Indianapolis, IN 46202 | (317) 274-7494